Where?

Next-Generation Sequencing Data Analysis
Quality Control,  Mapping, DNA-Seq  and RNA-Seq  Analysis

Organisers: RSBI (@RoBioinfo), Universitatea Politehnica Timișoara and West University of Timișoara

Where: the charming town of Timisoara

Preliminary dates: 15-16 November 2018

Participants: 10 MSc, Phd students and young scientists from Timisoara (10 places) and other main universities from Romania (up to 10 places). Once the program is finalized, up to 6  young participants from other East European countries will be invited.

A fundamental understanding of molecular biology  and basic knowledge of Linux, R and/ or Python are assumend. Prior to the event, resources on Unix, R and Python will be provided on the event webpage.

Proposed schedule & preliminary topics:

Day 1: NGS overview;  DNA-seq;

1. General introduction on  sequencing with focus on long read sequencing
   • RSBI member and or external speaker
2. DNA-seq methods: QC, assembly, annotation; mapping; variant calling; structural variants;
   • Speaker TDB
3. Keynote session (DNA-Seq perspectives)
   • Speaker TDB
4. SAMtools (possibly day 2)
   • Valeriu Ohan, Welcome Sanger Institute, UK

• Dinner and wine tasting

Day 2: RNA-seq

1. Bulk RNA sequencing analysis: quantifications; error models; analysis
   • Speaker TDB or RSBI member
2. Single cell RNA Seq
   • Speaker TDB
3. Keynote session (Single cell RNA-Seq)
   • Speaker TDB
4. 1-2 talks from senior RSBI members on topics pertaining to RNA-Seq
5. RNA-Seq perspectives
   • Speaker TBD

• Visit and diner at Recas winery or Timisoara by night